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The role of batokines in cardioprotection elicited by mild cold acclimation
Zvolská, Veronika ; Žurmanová, Jitka (advisor) ; Holzerová, Kristýna (referee)
Myocardial infarction remains one of the most serious diseases of civilization worldwide. The aim of the research is therefore to search for new strategies to protect the heart from ischemia-reperfusion (I/R) damage and its consequences. New findings can be used in clinical practice and for the prevention and treatment of heart diseases. This work builds on a study from our laboratory that developed a model of mild cold acclimation (5 weeks, 8 řC) associated with a significant reduction in infarct size after rat myocardial I/R without side effects. However, further study is needed to elucidate the mechanism of the cardioprotective effect. The diploma thesis was focused on the potential influence of known batokines, fibroblast growth factor (FGF21) and interleukin 6 (IL-6). Furthermore, we aimed to introduce a method for isolating adult rat cardiomyocytes and a procedure for verifying the viability and resistance of myocardial cells under the load of hypoxia and oxidative stress at the workplace. Effective cardioprotection of the mild cold acclimation was confirmed in the work. Acute ablation of BAT before the ischemia-reperfusion protocol had no effect on infarct size. The cardiomyocyte isolation method was successfully introduced and the protective effect of acute cold (10 days, 8 řC) was...
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Thyroid hormones, their importance and exposure to cold
Špínová, Šárka ; Žurmanová, Jitka (advisor) ; Krulová, Magdaléna (referee)
Thyroid hormones are essential mainly for prenatal development and they maintain the homeostasis of many organs and body processes in adulthood. Therefore, the correct function of the regulatory axis between the hypothalamus, pituitary gland and thyroid gland is very important. Lack of these hormones is manifested, for example, by the formation of goiter, cretenism and bradycardia, excess in weight loss, ophthalmopathy, excessive sweating, tachycardia and also goiter formation. A serious problem is also the mutation of thyroid hormone receptors in target tissues. It causes nervous system disorders, hearing, vision, digestive system, incorrect cardiac function, delayed growth and mental development. Thyroid hormones are essential for the formation and activation of brown adipose tissue. This, together with the shivering thermogenesis of skeletal muscle, is involved in facultative thermogenesis, helping homoeothermic mammals to maintain a constant body core temperature. In addition, brown adipose tissue is an important endocrine organ that secretes active substances called batokines upon cold activation. Batokines have beneficial effects in the treatment of obesity, diabetes and cardiovascular disease. So we can assume that cold adaptation could effectively help in the treatment of civilization...
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